Ecological and Evolutionary Functional Genomics (EuroEEFG)

Microbial Oceanography of ChemolithoAutotrophic planktonic Communities – MOCA

Abstract

Simulation models predict that the oxygen content of the global ocean will decrease by 25% until the end of the century due to an increased stratification of the oceanic surface waters and a rise in temperature. This loss in oxygen will inevitably lead to an expansion of hypoxic and anoxic areas in the global ocean with major consequences for the oceanic carbon and nitrogen cycling. In this proposal, we assess the functional diversity of chemolithoautotrophic prokaryotic communities in two contrasting marine environments, the deep-water masses of the North Atlantic along a latitudinal gradient and around the redoxcline in the central Baltic Sea. Both environments have been shown previously to harbor highly active chemolithoautotrophic prokaryotic communities with dark carbon dioxide fixation rates approaching surface water phytoplankton activity. Specific focus is put on the functional diversity of prokaryotes in the carbon and nitrogen cycling in both systems, including the sulfur cycle in the central Baltic. Biogeochemical rate measurements are tightly linked to functional gene analyses using among other approaches metagenomics and metatranscriptomics. Information obtained from these analyses will guide further in-depth studies of geochemically relevant processes in the water column of the two systems. Incubation experiments using stable and radio-isotopes in combination with molecular techniques such as SIP-RNA analyses, single-cell analyses using Raman-FISH, NanoSIMS and MICRO-FISH will allow insights into the dynamics of the functional diversity of chemolithotrophic microbial communities in suboxic and anoxic marine planktonic systems. Field studies will be complemented by laboratory model systems with isolated key players in order to understand the adaptive capacity and performance of chemolithoautotrophs in response to different environmental conditions. The combination of these approaches will provide the base for a significant advancement in our understanding of planktonic chemolithoautotrophy in the dark ocean.

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Project Participants

GerhardHerndlE-Mail
University of ViennaFaculty of Life ScienceDepartment of Marine BiologyViennaAustria
KlausJürgensE-Mail
Leibniz Institute of Baltic Sea ResearchDepartment of Biological OceanographyRostock-WarnemündeGermany
JaronePinhassiE-Mail
Linnaeus UniversitySchool Natural SciencesDepartment of Biology and Environmental ScienceMarine microbiologyKalmarSweden
ChristaSchleperE-Mail
University of ViennaFaculty of Life SciencesDepartment of Genetics in EcologyViennaAustria

Funding Agencies

  • Fonds zur Förderung der wissenschaftlichen Forschung in Österreich (FWF), Austria
  • Deutsche Forschungsgemeinschaft (DFG), Germany
  • Vetenskapsrådet (VR), Sweden

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