EuroSCOPE final report published

2 Reviews published in Microbial Cell Factories

Protein folding and conformational stress in microbial cells producing recombinant proteins: a host comparative overview

Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry

EuroSCOPE at the satellite symposium attached to the 13th European Biotechnology Congress (Barcelona ECB 13) - 16/19 September 2007

EuroSCOPE workshop on 18 September:

Production of membrane proteins
Prof. So Iwata, Director of the Centre for Structure Biology, Division of Molecular Biosciences, Imperial College London, United Kingdom

Stress response and protein production: Impact of the environment on complex protein secretion and host physiology (abstract)
Diethard Mattanovich, University of Natural Resources and Applied Life Sciences, Austria

Production and structural characterization of viral macromolecular complexes (abstract)
Dr. Roman Tuma, Reader, University of Leeds, UK

Towards a Bacillus cell factory for protein complexes and membrane proteins (abstract)
Prof. Jan Maarten van Dijl, Professor, Department of Pharmaceutical Biology, University of Groningen, The Netherlands

Interesting new properties of non-classical inclusion bodies (abstract)Mrs. Spela Peternel, Research Assistant, National Institute of Chemistry, Slovenia

A better understanding of the function of a protein requires a detailed analysis of its structure. Such studies (e.g. carried out on crystallized protein) require substantial amounts of high quality protein. The difficulties of producing sufficient amounts of protein for structure-function analysis as well as for x-ray analysis (crystallization) constituted thus far a major bottleneck for proteomics. Although this was and is well recognised by the scientific community, funding for a programme addressing this topic systematically has not been available since the beginning of the post-genomic phase that started proteomics. The EUROCORES programme EuroSCOPE bridges this gap by combining resources within Europe to accelerate research on protein production through scientific innovation and collaboration (more).


Development and Exploitation of Bacillus subtilis as a Host for the Production of Protein Complexesand Membrane Proteins

This project aims at developing new strategies for the production of multi-domain proteins and integral membrane proteins in Bacillus subtilis.

Project Leader: Jan Maarten van Dijl, University of Groningen, Groningen, Netherlands

Folding, Production and Assembly of Viral Complexes for High Resolution Structure Analysis

This project endeavours to first develop protein expression, folding and assembly systems that will then be used to produce viral complexes and to perform high resolution structural analyses of viral intermediary complexes.

Project Leader: Roman Tuma, University of Helsinki, Helsinki, Finland

Genome-Wide Comparison of Physiological Bottlenecks in Multi-Subunit Protein Production in Prokaryotic and Eukaryotic Microbial Hosts

The general concept of this project is a comparative (various host species with differing expression levels) genome-wide analysis of functional, structural and regulatory processes involved in the expression of complex proteins.

Project Leader: Diethard Mattanovich, University of Natural Resources and Applied Life Sciences, Vienna, Austria